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Registro Completo |
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
17/03/2017 |
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Data da última atualização: |
21/08/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
TURCHETTO, C.; GONZÁLES, H. H. S.; TORRES, G. A. M.; NHANI, A.; CONSOLI, L.; BINNECK, E. |
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Afiliação: |
GISELE ABIGAIL MONTAN TORRES, CNPT; ANTONIO NHANI JUNIOR, CNPT; LUCIANO CONSOLI, CNPT; ELISEU BINNECK, CNPSO. |
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Título: |
Suppression subtractive hybridization analysis of genes regulated by Magnaporthe oryzae infection in wheat adult plants. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE GENÉTICA, 61., 2015, Águas de Lindóia. Pós-genômica: [resumos]. [Ribeirão Preto]: Sociedade Brasileira de Genética, [2015]. |
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Páginas: |
p. 34. |
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Idioma: |
Inglês |
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Conteúdo: |
Blast (also known as brusone), caused by the fungus Magnaporthe oryzae, is a serious disease of wheat (Triticum aestivum) across central and southern Brazil. The pathogen is a hemibiotrophic ascomycete that attacks several grass species. The disease was first described in rice in 1600 in China and it was reported infecting wheat ears in 1985 in Paraná state, Brazil, and since spread to all growing-regions in the country. Currently has been also reported on wheat fields in Bolivia, Paraguay and Argentina. The rice blast disease has emerged as a model for the study of phytopathogenic fungi showing that this pathogen initially colonizes host tissues as a biotroph, without causing detectable symptoms. Approximately 72?96 h after infection, lesions become apparent in the plant, characterizing the necrotrophic growth of M. oryzae. In wheat plants, depending on the developmental stage at which infection occurs blast can be devastating. Infected heads produce small and wrinkled grains with low specific weight. Few cultivars are described as resistant to wheat blast and fungicides have low control efficiency of the disease. Little is known about the molecular mechanism of wheat resistance to the pathogen. Here, we investigated the responses of wheat to M. oryzae infection in reproductive stage at 40 h after inoculation. The aim of this study was to identify genes that are differentially up- or downregulated in adults plants of Triticum aestivum infected with Magnaporthe oryzae. For this, we used a suppression subtractive hybridization (SSH) approach. A total of 420 high-quality contigs were isolated, 415 of them were mapped in Triticum aestivum genome. The 420 contigs were searched against the non-redundant nucleotide and protein databases in GenBank to predict the function for the corresponding genes. Fifty-five contigs corresponded to defense-related genes. We used the quantitative RT-PCR analysis to validate the differential expression patterns for 16 Triticum aestivum genes between control and inoculated spikes. Nine genes presented higher transcript levels under inoculation, including one gene previously described as responsive to Magnaporthe infection on wheat seedlings. This gene coding one protein membrane-associated that may increase the adhesion of the plasma membrane to the cell wall during pathogen infection. In contrast, the other 7 genes presented higher expression in mock-inoculated spikes. The study of these genes and the associated defense mechanisms can provide a significant advance in our understanding of the putative determinants of the resistance mechanisms of this wheat resistant genotype. MenosBlast (also known as brusone), caused by the fungus Magnaporthe oryzae, is a serious disease of wheat (Triticum aestivum) across central and southern Brazil. The pathogen is a hemibiotrophic ascomycete that attacks several grass species. The disease was first described in rice in 1600 in China and it was reported infecting wheat ears in 1985 in Paraná state, Brazil, and since spread to all growing-regions in the country. Currently has been also reported on wheat fields in Bolivia, Paraguay and Argentina. The rice blast disease has emerged as a model for the study of phytopathogenic fungi showing that this pathogen initially colonizes host tissues as a biotroph, without causing detectable symptoms. Approximately 72?96 h after infection, lesions become apparent in the plant, characterizing the necrotrophic growth of M. oryzae. In wheat plants, depending on the developmental stage at which infection occurs blast can be devastating. Infected heads produce small and wrinkled grains with low specific weight. Few cultivars are described as resistant to wheat blast and fungicides have low control efficiency of the disease. Little is known about the molecular mechanism of wheat resistance to the pathogen. Here, we investigated the responses of wheat to M. oryzae infection in reproductive stage at 40 h after inoculation. The aim of this study was to identify genes that are differentially up- or downregulated in adults plants of Triticum aestivum infected with Magnaporthe oryzae. For t... Mostrar Tudo |
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Thesagro: |
Trigo. |
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Thesaurus Nal: |
Wheat. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/135952/1/suppression.binneck.2015.pdf
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Marc: |
LEADER 03393naa a2200217 a 4500
001 2067228
005 2019-08-21
008 2015 bl --- 0-- u #d
100 1 $aTURCHETTO, C.
245 $aSuppression subtractive hybridization analysis of genes regulated by Magnaporthe oryzae infection in wheat adult plants.$h[electronic resource]
260 $c2015
300 $ap. 34.
520 $aBlast (also known as brusone), caused by the fungus Magnaporthe oryzae, is a serious disease of wheat (Triticum aestivum) across central and southern Brazil. The pathogen is a hemibiotrophic ascomycete that attacks several grass species. The disease was first described in rice in 1600 in China and it was reported infecting wheat ears in 1985 in Paraná state, Brazil, and since spread to all growing-regions in the country. Currently has been also reported on wheat fields in Bolivia, Paraguay and Argentina. The rice blast disease has emerged as a model for the study of phytopathogenic fungi showing that this pathogen initially colonizes host tissues as a biotroph, without causing detectable symptoms. Approximately 72?96 h after infection, lesions become apparent in the plant, characterizing the necrotrophic growth of M. oryzae. In wheat plants, depending on the developmental stage at which infection occurs blast can be devastating. Infected heads produce small and wrinkled grains with low specific weight. Few cultivars are described as resistant to wheat blast and fungicides have low control efficiency of the disease. Little is known about the molecular mechanism of wheat resistance to the pathogen. Here, we investigated the responses of wheat to M. oryzae infection in reproductive stage at 40 h after inoculation. The aim of this study was to identify genes that are differentially up- or downregulated in adults plants of Triticum aestivum infected with Magnaporthe oryzae. For this, we used a suppression subtractive hybridization (SSH) approach. A total of 420 high-quality contigs were isolated, 415 of them were mapped in Triticum aestivum genome. The 420 contigs were searched against the non-redundant nucleotide and protein databases in GenBank to predict the function for the corresponding genes. Fifty-five contigs corresponded to defense-related genes. We used the quantitative RT-PCR analysis to validate the differential expression patterns for 16 Triticum aestivum genes between control and inoculated spikes. Nine genes presented higher transcript levels under inoculation, including one gene previously described as responsive to Magnaporthe infection on wheat seedlings. This gene coding one protein membrane-associated that may increase the adhesion of the plasma membrane to the cell wall during pathogen infection. In contrast, the other 7 genes presented higher expression in mock-inoculated spikes. The study of these genes and the associated defense mechanisms can provide a significant advance in our understanding of the putative determinants of the resistance mechanisms of this wheat resistant genotype.
650 $aWheat
650 $aTrigo
700 1 $aGONZÁLES, H. H. S.
700 1 $aTORRES, G. A. M.
700 1 $aNHANI, A.
700 1 $aCONSOLI, L.
700 1 $aBINNECK, E.
773 $tIn: CONGRESSO BRASILEIRO DE GENÉTICA, 61., 2015, Águas de Lindóia. Pós-genômica: [resumos]. [Ribeirão Preto]: Sociedade Brasileira de Genética, [2015].
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Registro original: |
Embrapa Trigo (CNPT) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Tabuleiros Costeiros. Para informações adicionais entre em contato com cpatc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
18/11/2008 |
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Data da última atualização: |
12/02/2009 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SANTOS, E. D. dos; RAMOS, S. R. R.; CARVALHO, H. W. L. de; MELO, K. E. de O. |
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Afiliação: |
Edwilson Dantas dos Santos, (estagiário) Embrapa Tabuleiros Costeiros; Semíramis Rabelo Ramalho Ramos, Embrapa Tabuleiros Costeiros; Hélio Wilson Lemos de Carvalho, Embrapa Tabuleiros Costeiros; Kátia Estelina de Oliveria Melo, (estagiária) Embrapa Tabuleiros Costeiros. |
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Título: |
Avaliação de híbridos de mandioca no estado de Sergipe. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 18; ENCONTRO DE PÓS-GRADUAÇÃO, 4., 2008, São Cristóvão. Anais... São Cristóvão: UFS, 2008. Ciências agrárias. 1 CD-ROM. |
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Idioma: |
Português |
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Palavras-Chave: |
Resumo. |
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Thesagro: |
Mandioca. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00592naa a2200169 a 4500
001 1372644
005 2009-02-12
008 2008 bl uuuu u00u1 u #d
100 1 $aSANTOS, E. D. dos
245 $aAvaliação de híbridos de mandioca no estado de Sergipe.
260 $c2008
650 $aMandioca
653 $aResumo
700 1 $aRAMOS, S. R. R.
700 1 $aCARVALHO, H. W. L. de
700 1 $aMELO, K. E. de O.
773 $tIn: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 18; ENCONTRO DE PÓS-GRADUAÇÃO, 4., 2008, São Cristóvão. Anais... São Cristóvão: UFS, 2008. Ciências agrárias. 1 CD-ROM.
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